Publications and Datasets
Publications and Datasets
Embedded Files
2025
2025
Oyarte Galvez L, Bisot C, Bourrianne P, Cargill R, Klein M, van Son M, van Krugten J, Caldas V, Clerc T, Lin KK, Kahane F, van Staalduine S, Stewart JD, Terry V, Turcu B, van Otterdijk S, Babu A, Kamp M, Seynen M, Steenbeek B, Zomerdijk J, Tutucci E, Sheldrake M, Godin C, Kokkoris V, Stone HA, Kiers ET, Shimizu TS. A travelling-wave strategy for plant-fungal trade. Nature (2025) doi: DOI: 10.1038/s41586-025-08614-x PDF
2024
2024
Preprint
Lattanzio MV, Sostaric N, Kanagasabesan N, Popovic B, Bradaric A, Wardak L, Guislain A, Savakis PE, Tutucci E* and Wolkers MC*. Single molecule imaging of transcription dynamics, RNA localization and fate in T cells BioRxiv (2024) doi:https://doi.org/10.1101/2024.11.11.622939 (*Co-corresponding author)
Updated Preprint
Maekiniemi A, Snoep SL, Seiler M, van Rossum K, van Niekerk DD, Savakis P, Zarnack K, Singer RH, Tutucci E*. Cyclin CLB2 mRNA localization determines efficient protein synthesis to orchestrate bud growth and cell cycle progression. BioRxiv (2024) doi: 10.1101/2022.03.01.481833 Preprint (*Corresponding author)
Botman D, Kanagasabapathi S, Rep M.I., van Rossum K, Tutucci E, Teusink B. cAMP in budding yeast: a messenger for sucrose metabolism? Biochim Biophys Acta Mol Cell Res. (2024); doi: 10.1016/j.bbamcr.2024.119706 PDF
Van Otterdijk S, Motealleh M, Wang Z, Visser T, Savakis P, Tutucci, E. Single-Molecule Fluorescent In Situ Hybridization (smFISH) for RNA Detection in the Fungal Pathogen Candida albicans MiMB (2024). doi:10.1007/978-1-0716-3766-1_2 PDF
2023
2023
Preprint
Botman D, Kanagasabapathi S, Rep M.I., van Rossum K, Tutucci E, Teusink B. cAMP in budding yeast: a messenger for sucrose metabolism? BioRxiv (2023); doi: https://doi.org/10.1101/2023.12.15.571809.
Dataset and analysis tools
Van Otterdijk S, Motealleh M, Wang Z, Visser T, Savakis P & Tutucci, E. Single-molecule Fluorescent In Situ Hybridization (smFISH) for RNA detection in the fungal pathogen Candida albicans small example dataset (2023). doi:10.5281/zenodo.8288705
Gerber A, van Otterdijk S, Bruggeman FJ, Tutucci, E*. Understanding spatiotemporal coupling of gene expression using single molecule RNA imaging technologies. Transcription. 2023; doi: 10.1080/21541264.2023.2199669. (*Corresponding author). PDF
2022
2022
Dataset and analysis tools
Meister L, Van Otterdijk S, Savakis P & Tutucci, E. Saccharomyces cerevisiae Bud-Annotation pipeline: Napari Example Data. (2022). doi:10.5281/zenodo.7004556
Tutucci E*, Maekiniemi A, Snoep SL, Seiler M, van Rossum K, van Niekerk DD, Savakis P, Zarnack K, Singer RH*. Cyclin CLB2 mRNA localization determines efficient protein synthesis to orchestrate bud growth and cell cycle progression. BioRxiv (2022) doi: 10.1101/2022.03.01.481833 Preprint (*Co-corresponding author)
2021
2021
Das, S, Vera, M, Gandin, V, Singer, RH*, Tutucci, E*. Intracellular mRNA transport and localized translation.
Nat Rev Mol Cell Biol. 2021;22 (7):483-504. doi: 10.1038/s41580-021-00356-8. (*Co-corresponding author). PDF
2020
2020
Tsuboi T, Viana MP, Xu F, Yu J, Chanchani R, Arceo XG, Tutucci E, Choi J, Chen YS, Singer RH, Rafelski SM, Zid BM. Mitochondrial volume fraction and translation duration impact mitochondrial mRNA localization and protein synthesis. eLife 2020 Aug 7;9:e57814 doi: 10.7554/eLife.57814 PDF
Pichon X, Robert MC, Bertrand E, Singer RH and Tutucci E*. New generations of MS2 variants and MCP fusions to detect single mRNAs in living eukaryotic cells. Methods in Molecular Biology – RNA tagging (July 2020) (*Corresponding author). 10.1007/978-1-0716-0712-1_7 PDF
Tutucci E* and Singer RH*. Simultaneous detection of mRNA and protein in S. cerevisiae by single molecule FISH and Immunofluorescence. Methods in Molecular Biology – RNA tagging (July 2020) (*Co-corresponding author). 10.1007/978-1-0716-0712-1_4 PDF
Dataset and analysis tools
Tutucci E, Maekiniemi A, Singer RH. (2020) Dataset related to the publication: “Single molecule mRNA Fluorescent In Situ Hybridization combined to Immunofluorescence in S. cerevisiae: Dataset and quantification”, VU Amsterdam, V5, https://doi.org/10.17632/bcmn9cxyzs.4
Maekiniemi A, Singer RH, Tutucci E*. Single molecule mRNA fluorescent in situ hybridization combined with immunofluorescence in S. cerevisiae: Dataset and quantification (April 2020) (*Corresponding author). https://doi.org/10.1016/j.dib.2020.105511 PDF
2019
2019
Vera M, Tutucci E, and Singer RH. Imaging single mRNA molecules in mammalian cells using an optimized MS2-MCP system. Methods in Molecular Biology – Imaging Gene Expression (2019). doi: 10.1007/978-1-4939-9674-2_1 PDF
Infantino V*, Tutucci E*, Bagdiul I, Palancade B, Yeh Martin N, Zihlman A, Garcia-Molinero V, Silvano G and Stutz F. The mRNA export adaptor Yra1 contributes to DNA double-strand break repair through its C-box domain. PloS one 14 (4), e0206336 (2019) (*Equal contribution) https://doi.org/10.1371/journal.pone.0206336 PDF
Tsuboi T, Viana MP, Xu F, Yu J, Chanchani R, Arceo XG, Tutucci E, Choi J, Chen YS, Singer RH, Rafelski SM, Zid BM. Mitochondrial volume fraction controls translation of nuclear-encoded mitochondrial proteins. BioRxiv (January 1st, 2019) https://doi.org/10.1101/529289 Pre-print
2018
2018
Infantino V*, Tutucci E*, Bagdiul I, Palancade B, Yeh Martin N, Zihlman A, Garcia-Molinero V, Silvano G and Stutz F. The mRNA export adaptor Yra1 contributes to DNA double-strand break repair through its C-box domain (*Equal contribution). BioRxiv (October 15th, 2018). https://doi.org/10.1101/441980 Preprint
Tutucci E, Vera M and Singer RH, Single mRNA detection in living S. cerevisiae using a re-engineered MS2 system. Nature Protocols (September 14, 2018) https://doi.org/10.1038/s41596-018-0037-2 PDF
Tutucci E and Singer RH Shining light on the demise of single mRNAs. Cell Systems, Invited commentary for the Series Principles of Systems Biology (No. 26) (February 28, 2018) https://doi.org/10.1016/j.cels.2018.02.002 PDF
Tutucci E, Livingston N, Wu B, Singer RH Imaging mRNA In Vivo, from birth to death. Annual review of biophysics 47 (2018) https://doi.org/10.1146/annurev-biophys-070317-033037 PDF
Tutucci E*, Vera M*, Biswas J, Garcia J, Parker R and Singer RH. An improved MS2 system for accurate reporting of the mRNA life cycle. Nature methods 15, 81-89 (January 2018) (*Equal contribution) https://doi.org/10.1038/nmeth.4502 PDF
2016
2016
Brickner DG, Sood V, Tutucci E, Coukos R, Viets K, Singer RH, Brickner JH. Subnuclear positioning and interchromosomal clustering of the GAL1-10 locus are controlled by separable, interdependent mechanisms Mol Biol Cell 27(19):2980-2993 (2016 October 1). https://doi.org/10.1091/mbc.E16-03-0174 PDF
2015
2015
Wu B, Miskolci V, Sato H, Tutucci E, Kenworthy CA, Donnelly SK, Yoon YJ, Cox D, Singer RH, Hodgson L. Synonymous modification results in high-fidelity gene expression of repetitive protein and nucleotide sequences. Genes & development 29(8):876-886, April 2015 https://doi.org/10.1101/gad.259358.115 PDF
2011
2011
Tutucci E, Stutz F. Keeping mRNPs in check during assembly and nuclear export. Nat Rev Mol Cell Biol. 2011 Jun;12(6):377-84 https://doi.org/10.1038/nrm3119 PDF
2010
2010
Iglesias N*, Tutucci E*, Gwizdek C, Vinciguerra P, Von Dach E, Corbett AH, Dargemont C, Stutz F. Ubiquitin-mediated mRNP dynamics and surveillance prior to budding yeast mRNA export. Genes Dev. 2010 Sep 1;24(17):1927-38; https://doi.org/10.1101/gad.583310 (*Equal contribution) PDF
Terrile M, Appolloni I, Calzolari F, Perris R, Tutucci E, Malatesta P. PDGF-B-driven gliomagenesis can occur in the absence of the proteoglycan NG2. BMC Cancer. 2010 Oct 12; 10:550 https://doi.org/10.1186/1471-2407-10-550 PDF
2009
2009
Appolloni I, Calzolari F, Tutucci E, Caviglia S, Terrile M, Corte G, Malatesta P. PDGF-B induces a homogeneous class of oligodendrogliomas from embryonic neural progenitors. Int J Cancer. 2009 May 15;124(10):2251-9; https://doi.org/10.1002/ijc.24206 PDF
2008
2008
Calzolari F, Appolloni I, Tutucci E, Caviglia S, Terrile M, Corte G, Malatesta P. Tumor progression and oncogene addiction in a PDGF-B-induced model of gliomagenesis. Neoplasia. 2008 Dec; https://doi.org/10.1593/neo.08814 PDF
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