Publications

2022

Tutucci E*, Maekiniemi A, Snoep SL, Seiler M, van Rossum K, van Niekerk DD, Savakis P, Zarnack K, Singer RH*. Cyclin CLB2 mRNA localization determines efficient protein synthesis to orchestrate bud growth and cell cycle progression. BioRxiv (March 2nd, 2022) doi: 10.1101/2022.03.01.481833 Preprint (*Co-corresponding author)

2021

Das, S, Vera, M, Gandin, V, Singer, RH*, Tutucci, E*. Intracellular mRNA transport and localized translation.

Nat Rev Mol Cell Biol. 2021;22 (7):483-504. doi: 10.1038/s41580-021-00356-8. (*Co-corresponding author). PDF

2020

Tsuboi T, Viana MP, Xu F, Yu J, Chanchani R, Arceo XG, Tutucci E, Choi J, Chen YS, Singer RH, Rafelski SM, Zid BM. Mitochondrial volume fraction and translation duration impact mitochondrial mRNA localization and protein synthesis. eLife 2020 Aug 7;9:e57814 doi: 10.7554/eLife.57814 PDF

Pichon X, Robert MC, Bertrand E, Singer RH and Tutucci E*. New generations of MS2 variants and MCP fusions to detect single mRNAs in living eukaryotic cells. Methods in Molecular Biology – RNA tagging (July 2020) (*Corresponding author). 10.1007/978-1-0716-0712-1_7 PDF

Tutucci E* and Singer RH*. Simultaneous detection of mRNA and protein in S. cerevisiae by single molecule FISH and Immunofluorescence. Methods in Molecular Biology – RNA tagging (July 2020) (*Co-corresponding author). 10.1007/978-1-0716-0712-1_7 PDF

Maekiniemi A, Singer RH, Tutucci E*. Single molecule mRNA fluorescent in situ hybridization combined with immunofluorescence in S. cerevisiae: Dataset and quantification (April 2020) (*Corresponding author). https://doi.org/10.1016/j.dib.2020.105511 PDF

2019

Vera M, Tutucci E, and Singer RH. Imaging single mRNA molecules in mammalian cells using an optimized MS2-MCP system. Methods in Molecular Biology – Imaging Gene Expression (2019). doi: 10.1007/978-1-4939-9674-2_1 PDF

Infantino V*, Tutucci E*, Bagdiul I, Palancade B, Yeh Martin N, Zihlman A, Garcia-Molinero V, Silvano G and Stutz F. The mRNA export adaptor Yra1 contributes to DNA double-strand break repair through its C-box domain. PloS one 14 (4), e0206336 (2019) (*Equal contribution) https://doi.org/10.1371/journal.pone.0206336 PDF

Tsuboi T, Viana MP, Xu F, Yu J, Chanchani R, Arceo XG, Tutucci E, Choi J, Chen YS, Singer RH, Rafelski SM, Zid BM. Mitochondrial volume fraction controls translation of nuclear-encoded mitochondrial proteins. BioRxiv (January 1st, 2019) https://doi.org/10.1101/529289 Pre-print

2018

Infantino V*, Tutucci E*, Bagdiul I, Palancade B, Yeh Martin N, Zihlman A, Garcia-Molinero V, Silvano G and Stutz F. The mRNA export adaptor Yra1 contributes to DNA double-strand break repair through its C-box domain (*Equal contribution). BioRxiv (October 15th, 2018). https://doi.org/10.1101/441980 Preprint

Tutucci E, Vera M and Singer RH, Single mRNA detection in living S. cerevisiae using a re-engineered MS2 system. Nature Protocols (September 14, 2018) https://doi.org/10.1038/s41596-018-0037-2 PDF

Tutucci E and Singer RH Shining light on the demise of single mRNAs. Cell Systems, Invited commentary for the Series Principles of Systems Biology (No. 26) (February 28, 2018) https://doi.org/10.1016/j.cels.2018.02.002 PDF

Tutucci E, Livingston N, Wu B, Singer RH Imaging mRNA In Vivo, from birth to death. Annual review of biophysics 47 (2018) https://doi.org/10.1146/annurev-biophys-070317-033037 PDF

Tutucci E*, Vera M*, Biswas J, Garcia J, Parker R and Singer RH. An improved MS2 system for accurate reporting of the mRNA life cycle. Nature methods 15, 81-89 (January 2018) (*Equal contribution) https://doi.org/10.1038/nmeth.4502 PDF

2016

Brickner DG, Sood V, Tutucci E, Coukos R, Viets K, Singer RH, Brickner JH. Subnuclear positioning and interchromosomal clustering of the GAL1-10 locus are controlled by separable, interdependent mechanisms Mol Biol Cell 27(19):2980-2993 (2016 October 1). https://doi.org/10.1091/mbc.E16-03-0174 PDF

2015

Wu B, Miskolci V, Sato H, Tutucci E, Kenworthy CA, Donnelly SK, Yoon YJ, Cox D, Singer RH, Hodgson L. Synonymous modification results in high-fidelity gene expression of repetitive protein and nucleotide sequences. Genes & development 29(8):876-886, April 2015 https://doi.org/10.1101/gad.259358.115 PDF

2011

Tutucci E, Stutz F. Keeping mRNPs in check during assembly and nuclear export. Nat Rev Mol Cell Biol. 2011 Jun;12(6):377-84 https://doi.org/10.1038/nrm3119 PDF

2010

Iglesias N*, Tutucci E*, Gwizdek C, Vinciguerra P, Von Dach E, Corbett AH, Dargemont C, Stutz F. Ubiquitin-mediated mRNP dynamics and surveillance prior to budding yeast mRNA export. Genes Dev. 2010 Sep 1;24(17):1927-38; https://doi.org/10.1101/gad.583310 (*Equal contribution) PDF

Terrile M, Appolloni I, Calzolari F, Perris R, Tutucci E, Malatesta P. PDGF-B-driven gliomagenesis can occur in the absence of the proteoglycan NG2. BMC Cancer. 2010 Oct 12; 10:550 https://doi.org/10.1186/1471-2407-10-550 PDF

2009

Appolloni I, Calzolari F, Tutucci E, Caviglia S, Terrile M, Corte G, Malatesta P. PDGF-B induces a homogeneous class of oligodendrogliomas from embryonic neural progenitors. Int J Cancer. 2009 May 15;124(10):2251-9; https://doi.org/10.1002/ijc.24206 PDF

2008

Calzolari F, Appolloni I, Tutucci E, Caviglia S, Terrile M, Corte G, Malatesta P. Tumor progression and oncogene addiction in a PDGF-B-induced model of gliomagenesis. Neoplasia. 2008 Dec; https://doi.org/10.1593/neo.08814 PDF