We study how microbes control gene expression in space and time to shape their physiology and survival. Our team develops imaging and analysis tools to track RNA molecules inside single cells with molecular precision, across both model and medically or industrially relevant microbes.
By integrating high-resolution imaging with molecular biology and genetics, we study how microbes localize RNA molecules within subcellular compartments to control protein synthesis and growth. Ultimately, our goal is to uncover the decision-making strategies that enable cells to adapt to dynamic environments—both as individuals and as coordinated communities—to maximize survival and fitness.
We love microbes!
S. cerevisiae
Filamentous S. cerevisiae
Candida albicans
Arbuscular mycorrhizal fungi
Aspergillus niger
E. coli
We are always open to explore the physiology of new microorganisms. Contact us for collaborations!
1 - Methods development for RNA visualization and imaging analysis in fixed microbial cells based on single molecule RNA FISH (smFISH)
smFISH-IF in S. cerevisiae
2 - Methods development for RNA imaging in live microbial cells based on the MS2 and PP7 systems
Live imaging MS2 tagged mRNAs Maekiniemi et al. 2024
3 - Regulation of filamentous fungi differentiation and biofilm formation
Candida albicans
4 - RNA localization and spatial control of protein synthesis
5 - Regulation of mRNA degradation in S. cerevisiae
6 - Development of imaging analysis pipelines for microbial species
Contact evelina.tutucci[at]vu.nl to get more information on the projects
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